A genetic algorithm for robust berth allocation and quay crane assignment

Scheduling problems usually obtain the optimal solutions assuming that the environment is deterministic. However, actually the environment is dynamic and uncertain. Thus, the initial data could change and the initial schedule obtained might be unfeasible. To overcome this issue, a proactive approach is presented for scheduling problems without any previous knowledge about the incidences that can occur. In this paper, we consider the berth allocation problem and the quay crane assignment problem as a representative example of scheduling problems where a typical objective is to minimize the service time. The robustness is introduced within this problem by means of buffer times that should be maximized to absorb possible incidences or breakdowns. Therefore, this problem becomes a multi-objective optimization problem with two opposite objectives: minimizing the total service time and maximizing the robustness or buffer time

Robustness for a single railway line: Analytical and simulation methods

[EN] Railway scheduling has been a significant issue in the railway industry. Over the last few years, numerous approaches and tools have been developed to compute railway scheduling. However, robust solutions are necessary to absorb short disruptions. In this paper, we present the robustness problem from the point of view of railway operators and we propose analytical and simulation methods to measure robustness in a single railway line. In the analytical approach, we have developed some formulas to measure robustness based on the study of railway line infrastructure topology and buffer times. In the simulation approach, we have developed a software tool to assess the robustness for a given schedule. These methods have been inserted in MOM (More information can be found at the MOM web page http://www.dsic.upv.es/users/ia/gps/MOM), which is a project in collaboration with the Spanish Railway Infrastructure Manager (ADIF). © 2012 Elsevier Ltd. All rights reserved.This work has been partially supported by the research project TIN2010-20976-C02-01 (Min. de Economia y Competitividad, Spain) and project PIRSES-GA-2011-294931 (FP7-PEOPLE-2011-IRSES).Salido Gregorio, MA.; Barber Sanchís, F.; Ingolotti Hetter, LP. (2012). Robustness for a single railway line: Analytical and simulation methods. Expert Systems with Applications. 39(18):13305-13327. https://doi.org/10.1016/j.eswa.2012.05.071S1330513327391

Reversal of gastrointestinal carcinoma-induced immunosuppression and induction of antitumoural immunity by a combination of cyclophosphamide and gene transfer of IL-12

Immunotherapy-based strategies for gastrointestinal carcinomas (GIC) have been exploited so far, but these approaches have to face strong mechanisms of immune escape induced by tumours. We previously demonstrated that sub-therapeutic doses of an adenovirus expressing IL-12 genes (AdIL-12) mediated a potent antitumour effect against subcutaneous (s.c.) colorectal carcinomas (CRC) in mice pre-treated with low doses of cyclophosphamide (Cy). In our study we used this combination to assess its impact on the immunosuppressive microenvironment. In s.c. CRC model we demonstrated that non-responder mice failed to decrease Tregs in tumour, spleen and peripheral blood. Reconstitution of Tregs into tumour-bearing mice treated with combined therapy abolished the antitumoural effect. In addition, Cy + AdIL-12 modified Tregs functionality by inhibiting the in vitro secretion of IL-10 and TGF-β and their ability to inhibit dendritic cells activation. Combined treatment decreased the number of myeloid-derived suppressor cells (MDSCs) in comparison to non-treated mice and, interestingly, administration of Tregs restored splenic MDSCs population. Furthermore, combined therapy potently generated specific cytotoxic IFN-γ-secreting CD4+ T cells able to eradicate established CRC tumours after adoptive transfer. Finally, we evaluated the combination on disseminated CRC and pancreatic carcinoma (PC). Cy + AdIL-12 were able to eradicate liver metastatic CRC (47%) and PC tumour nodules (40%) and to prolong animal survival. The results of this study support the hypothesis that Cy + AdIL-12 might be a valid immunotherapeutic strategy for advanced GIC.Fil: Malvicini, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Austral; ArgentinaFil: Ingolotti, Mariana. Universidad Austral; ArgentinaFil: Piccioni, Flavia Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Austral; ArgentinaFil: García, Mariana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Austral; ArgentinaFil: Bayo Fina, Juan Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Austral; ArgentinaFil: Atorrasagasti, María Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Austral; ArgentinaFil: Alaniz, Laura Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Austral; ArgentinaFil: Aquino, Jorge Benjamin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Austral; ArgentinaFil: Espinoza, Jaime A.. Universidad Adolfo Ibañez; Chile. Universidad de La Frontera; ChileFil: Gidekel, Manuel. Universidad Adolfo Ibañez; ChileFil: Scharovsky, Olga Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Medicas. Instituto de Genetica Experimental; ArgentinaFil: Matar, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Medicas. Instituto de Genetica Experimental; ArgentinaFil: Mazzolini Rizzo, Guillermo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Austral; Argentin

An Efficient Method to Schedule New Trains on a Heavily Loaded Railway Network

With the aim of supporting the process of adapting railway infrastructure to present and future traffic needs, we have developed a method to build train timetables efficiently. In this work, we describe the problem in terms of constraints derived from railway infrastructure, user requirements and traffic constraints, and we propose a method to solve it efficiently. This method carries out the search by assigning values to variables in a given order and verifying the satisfaction of constraints where these are involved. When a constraint is not satisfied, a guided backtracking is done. The technique reduces the search space allowing us to solve real and complex problems efficiently

Ocular Myasthenia Gravis: AChR Seropositivy and Generalization

Ocular Myasthenia Gravis (OMG) is an autoimmune disorder of the neuromuscular junction characterized by fatigable weakness of extraocular muscles, levator palpebrae, and orbicularis oculi resulting in fatigable ptosis and binocular diplopia. The predominant auto-antibodies in Myasthenia Gravis are, among others, those against acethylcholine receptors. Multiples studies reported a seropositivity between 40% and 60% f or OMG. Some patients may develop weakness in their limbs, bulbar or respiratory muscles,that is, Generalized Myasthenia Gravis (GMG). The purpose of this study is to report the frequency of Acetylcholine Receptor Antibody (AChR) seropositivity i n patients with clinical suspicion of OMG and the percentage of these patients who developed GMG during the follow up